Levofloxacin is a broad-spectrum fluoroquinolone antibiotic frequently used to treat both acute and chronic bacterial prostatitis. It works by inhibiting bacterial DNA gyrase and topoisomerase IV, thereby preventing bacterial replication and repair. Levofloxacin achieves excellent penetration into prostate tissue, making it effective against common pathogens such as E. coli, Klebsiella, and Proteus species. Typical treatment duration is 2–4 weeks for acute infections and up to 6 weeks for chronic prostatitis. Clinicians monitor for potential adverse effects—including tendon inflammation, QT prolongation, CNS effects, and antibiotic resistance—especially in older adults or those with comorbidities.